- The Effect of Methimazole on the Thyroglobulin Synthesis in Cultured Porcine Thyroid Cells.
-
Eun Jig Lee, Hyun Chul Lee, Kap Bum Huh, Kyung Rae Kim, Sung Kil Lim, Kyung Mi Lee
-
J Korean Endocr Soc. 1994;9(4):332-336. Published online November 6, 2019
-
-
-
 Abstract
 PDF
- The thioureylene drugs, propylthiouracil and methylmercaptoimidazol(MMI), exert their antithyroid effect primarily through inhibition of thyroid peroxidase-catalyzed iodination of thyroglobulin. Recently the interest about the effect to the thyroglobulin synthesis of these drugs have been increasing. So we studied the MMI effect to the thyroglobulin synthesis in cultured porcine thyroid cells. Porcine thyroid cells were isolated by sequential trypsinization in the presence of EGTA, seeded at high density(1X10^6 cells/cm^2) and cultured. One week later, MMI was added in different concentrations(0, 0.2, 1, 5mM) with TSH only or with 4H(b-TSH, Insulin, Transferrin, Hydrocortisone) or without hormone. Medias were collected after 24 hours and compared the amount of thyroglobulin secreted. And also pulse-labeling were performed with S^35 cysteine/methionine(1-2uCi/well) for 30, 60, 90min at the same conditions.There was no significant change in the amount of the secreted thyroglobulin by MMI, and there was no significant change in the pulse-labeled interacellular thyroglobulin by MMI. And also there was no significant change in the secretion of TSH-stimulated thyroglobulin by MMI. So we conclude that MMI has no effect on the thyroglobulin synthesis in cultured porcine thyroid cells and also MMI has no effect on the TSH-stimulated thyroglobulin synthesis in cultured porcine thyroid cell.
- The Effect of Iodine on the thyroglobulin Synthesis in Cultured Porcine Thyroid Cells.
-
Eun Jig Lee, Hyun Chul Lee, Kap Bum Huh, Kyung Rae Kim, Sung Kil Lim, Kyung Mi Lee
-
J Korean Endocr Soc. 1994;9(4):318-324. Published online November 6, 2019
-
-
-
Abstract
PDF
- The amount of thyroglobulin synthesized from thyroid cells and stored in colloid space in very important in thyroid hormone synthesis. The thyroglobulin synthesis is mainly regulated by TSH secreted from the pituitary gland. But recently there were some reports about the possibility that iodine regulated the thyroid protein synthesis. So our studied were conducted to determined whether iodine could have inhibitory effect on thyroglobulin synthesis and methimazole could abolish the inhibitory effect of idoine.Porcine thyroid cells were isolated by sequential trypsinization in the presence of EGTA, seeded at high density(1X10^6 cells/cm^2) and cultured. One week later, Nal was added in different concentrations(10^-7, 10^-6, 10^-5, 10^-4M). 24hour medias were collected and checked the amount of thyroglobulin secreted. And also pulse-labeling were performed with[^35S] cysteine/methionine(1-2 uCi/well) for 1 hour at the same conditions. We used 3mM methimazole and 10^-4M NaI to observe the blocking effect of methimazol in iodine.The extracellular thyroglobulin secretion was significantly decreased by iodine in dose dependent manner(82.4%, 80.7%, 76.8% and 73.1% of control). And also intracellular thyroglobulin synthesis was significantly decreased by iodide in dose dependent manner(100.5%, 83.4%, 82.3% and 79.4% of control). The inhibitory effect of iodide was abolished by methimazole(74.7% to 101.3% of control). These data indicate that high iodide inhibit the thyroglobulin synthesis and secretion from the thyroid cells, and furthermore autoregulation by iodide may include thyroglobulin synthesis. And also this effect is dependent on the generation of an organic form of iodine because methimazole abolish the inhibitory effect of iodide.
- A case of cushing's syndrome due to bilateral macronodular adrenocortical hyperplasia associated with empty sella syndrome.
-
Jin Ahn Kim, Kyung Mi Lee, Yoon Sok Chung, Eun Tack Shin, Uk Hee Won, Eun Jig Lee, Kyung Rae Kim, Hyun Chul Lee, Kap Bum Huh, Ki Whang Kim, Hee De Lee, Woo Hee Jung
-
J Korean Endocr Soc. 1993;8(2):203-210. Published online January 1, 2001
-
-
-
Abstract
PDF
- No abstract available.
- The correlation between body composition and bone mineral density in pre-and postmenopausal women.
-
Moon Suk Nam, Eun Jig Lee, Kyung Rae Kim, Kyung Mi Lee, Yoon Sok Chung, Sung Kil Lim, Hyun Chul Lee, Byung Suk Lee, Ki Hyun Park, Kap Bum Huh
-
J Korean Endocr Soc. 1993;8(2):180-186. Published online January 1, 2001
-
-
-
Abstract
PDF
- No abstract available.
- Comparision of bone mineral density in acromegalic pateints according to the gonadal status.
-
Seog Won Park, Young Soo Kim, Woon Sok Chung, Kyung Mi Lee, Eun Jig Lee, Sung Kil Lim, Kyung Rae Kim, Hyun Chul Lee, Kap Bum Huh
-
J Korean Endocr Soc. 1993;8(2):149-156. Published online January 1, 2001
-
-
-
Abstract
PDF
- No abstract available.
- A case of pituitary metastasis from periampullary carcinoma.
-
Eun Tack Shin, Eun Jig Lee, Kyung Rae Kim, Kyung Mi Lee, Hee Dong Bae, Kwan Sik Lee, Yoon Sok Chung, Kwang Jin Ahn, Sung Kil Lim, Hyun Chul Lee, Kap Bum Huh
-
J Korean Endocr Soc. 1993;8(1):88-93. Published online January 1, 2001
-
-
-
Abstract
PDF
- No abstract available.
- Thyrotropin secreating pituitary adenoma.
-
Eun Jig Lee, Kyung Rae Kim, Hyeon Man Kim, Yoon Sok Chung, Kwang Jin Ahn, Kyung Mi Lee, Sung Kil Lim, Hyun Chul Lee, Duk Hi Kim, Dong Ik Kim, Doe Heum Yoon, Yong Gu Park
-
J Korean Endocr Soc. 1992;7(4):331-342. Published online January 1, 2001
-
-
-
Abstract
PDF
- No abstract available.
|
KES
